{"id":209,"date":"2015-05-06T06:27:26","date_gmt":"2015-05-06T06:27:26","guid":{"rendered":"http:\/\/www.altmann.eu\/research\/?p=209"},"modified":"2015-05-29T22:58:10","modified_gmt":"2015-05-29T22:58:10","slug":"paper-accepted-in-plos-one","status":"publish","type":"post","link":"http:\/\/www.altmann.eu\/research\/?p=209","title":{"rendered":"Paper accepted in PLoS ONE"},"content":{"rendered":"<p>Our paper with the tile &#8220;<a href=\"http:\/\/journals.plos.org\/plosone\/article?id=10.1371\/journal.pone.0128465\"><em><strong>Connecting Anxiety and Genomic Copy Number Variation: a Genome-Wide Analysis in Mice<\/strong><\/em><\/a>&#8221; was accepted for publication at PLoS ONE.<\/p>\n<p><strong>Abstract:<\/strong><\/p>\n<div class=\"page\" title=\"Page 1\">\n<div class=\"section\">\n<div class=\"layoutArea\">\n<div class=\"column\">\n<p>Genomic copy number variants (CNVs) have been implicated in multiple psychiatric disorders, but not much is known about their influence on anxiety disorders specifically. Using next-generation sequencing (NGS) and two additional array-based genotyping approaches, we detected CNVs in a mouse model consisting of two inbred mouse lines showing high (HAB) and low (LAB) anxiety-related behavior, respectively. An influence of CNVs on gene expression in the central (CeA) and basolateral (BLA) amygdala, paraventricular nucleus (PVN), and cingulate cortex (Cg) was shown by a two-proportion\u00a0<em>Z<\/em>-test (<em>p<\/em>\u00a0= 1.6 x 10-31), with a positive correlation in the CeA (<em>p<\/em>\u00a0= 0.0062), PVN (<em>p<\/em>\u00a0= 0.0046) and Cg (<em>p<\/em>\u00a0= 0.0114), indicating a contribution of CNVs to the genetic predisposition to trait anxiety in the specific context of HAB\/LAB mice. In order to confirm anxiety-relevant CNVs and corresponding genes in a second mouse model, we further examined CD-1 outbred mice. We revealed the distribution of CNVs by genotyping 64 CD 1 individuals using a high-density genotyping array (Jackson Laboratory). 78 genes within those CNVs were identified to show nominally significant association (48 genes), or a statistical trend in their association (30 genes) with the time animals spent on the open arms of the elevated plus-maze (EPM). Fifteen of them were considered promising candidate genes of anxiety-related behavior as we could show a significant overlap (permutation test,\u00a0<em>p<\/em>\u00a0= 0.0051) with genes within HAB\/LAB CNVs. Thus, here we provide what is to our knowledge the first extensive catalogue of CNVs in CD-1 mice and potential corresponding candidate genes linked to anxiety-related behavior in mice.<\/p>\n<\/div>\n<\/div>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>Our paper with the tile &#8220;Connecting Anxiety and Genomic Copy Number Variation: a Genome-Wide Analysis in Mice&#8221; was accepted for publication at PLoS ONE. Abstract: Genomic copy number variants (CNVs) have been implicated in multiple psychiatric disorders, but not much is known about their influence on anxiety disorders specifically. Using next-generation sequencing (NGS) and two [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[7],"tags":[],"class_list":["post-209","post","type-post","status-publish","format-standard","hentry","category-research","entry"],"_links":{"self":[{"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=\/wp\/v2\/posts\/209","targetHints":{"allow":["GET"]}}],"collection":[{"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=209"}],"version-history":[{"count":2,"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=\/wp\/v2\/posts\/209\/revisions"}],"predecessor-version":[{"id":222,"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=\/wp\/v2\/posts\/209\/revisions\/222"}],"wp:attachment":[{"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=209"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=209"},{"taxonomy":"post_tag","embeddable":true,"href":"http:\/\/www.altmann.eu\/research\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=209"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}